The metabolic degradation in the mouse of 1,2,5,6-dibenzanthracene-9,10-C14. II. 5-Hydroxy-1,2-naphthalic acid, a new metabolite.

pentacyclic aromatic ring, and any metabolite resulting from cleavage or alteration of the aro matic character of the hydrocarbon could not be identified unequivocally by this analytical method. Another technic for these studies was made avail able following the synthesis of 1,2,5,6-dibenzanthracene-9,10-C14(9) ; and by measuring the radio activity of tissues and their fractions following the administration of the labeled carcinogen to mice, Heidelberger et al. (10, 11) were able to obtain quantitative distributions of radioactivity and to demonstrate the production of acidic metabolites.